Cytokeratin 1 anticorps (pan)

N° du produit ABIN356300

L’anticorps Souris Monoclonal anti-Cytokeratin 1 a été validé pour IHC (p), IF et IHC (fro). Il convient pour détecter Cytokeratin 1 dans des échantillons de Humain, Rat, Souris, Boeuf (Vache), Lapin, Poulet, Singe et Porc.

Aperçu rapide pour Cytokeratin 1 anticorps (pan) (ABIN356300)

Antigène: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Lapin, Poulet, Singe, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AE1-AE3

Détail du produit anti-Cytokeratin 1 anticorps

Épitope: pan

Specificité: AE 1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors, reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins). This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. This antibody AE 1/AE 3 recognizes Low Molecular Weight Cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40) of the acidic family and CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5).
Cellular Localization: Cytoplasmic.Cellular Localization:

Réactivité croisée (Details): Species reactivity (tested):Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.

Attributs du produit: Synonyms: pan Keratin, Cytokeratin Pan-reactive

Purification: Protein A Chromatography.

Immunogène: Human epidermal keratin. Remarks: Molecular weight of antigen: CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59), CK5 (58)CK6 (56) CK8 (52. 5). + CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) CK8 (52. 5).

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentrated antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. For FFPE tissue sections antigen retriever like trypsin is required. Recommended Positive Control: Human skin, lung ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur Cytokeratin 1

Antigène: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Autre désignation: Cytokeratin

Sujet: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).Synonyms: Cytokeratin pan-reactive, pan Keratin